Two-cells-in-one combo therapy could bolster leukemia treatment
A new delivery system guides drugs directly to cancer cells in bone marrow
Science Daily, October 31, 2018
This is a scanning electron microscope colorized to show the hematopoietic stem cells (purple) and blood platelets (green) joined together.
Credit: Zhen Gu Research Lab/UCLA Samueli.
A cancer therapy based on fusing two types of cells into a single unit shows promise in strengthening existing treatments for acute myeloid leukemia. The approach joins blood platelets that carry cancer drugs with stem cells that guide the platelets into bone marrow where leukemia begins.
Researchers found that when injected into mice that had acute myeloid leukemia, the combination therapy halted the disease from developing any further. Of the mice that received the treatment, 87.5 percent were cured by 80 days after the combination cells were injected. Those mice also were all resistant to leukemia cells that were re-injected two months after the 80-day period.
The study was published in Nature Biomedical Engineering.
Zhen Gu, a professor of bioengineering at the UCLA Samueli School of Engineering who led the study, said the approach could be used in concert with other therapies, such as chemotherapy and stem cell treatment, to improve their effectiveness. Gu said the method would have to be tested and approved in human clinical trials before it could be incorporated in treatments for people with leukemia.
Acute myeloid leukemia is a cancer that starts in bone marrow and can spread to the bloodstream and other parts of the body. With a compromised immune system, a person with this type of leukemia could die from complications from other diseases.
As a treatment for leukemia, chemotherapy on its own is only moderately effective: Leukemia fails to go into remission in about 1 in 3 patients following chemotherapy, according to the American Cancer Society. And about half of people with the disease who do experience remission may have a relapse -- typically within two years after treatment -- usually because chemotherapy cannot reach cancer cells in bone marrow.
The UCLA-led research aimed to solve that problem by devising a method to deliver medicine directly into the bone marrow. The approach, termed "cell combination drug delivery," is the first to link two different cells together for therapeutic purposes.
In the combined cells, the blood platelets are used to deliver immunotherapy drugs called checkpoint inhibitors (the UCLA researchers used a drug called an aPD-1 antibody), which seek out cancer cells and neutralize their defenses. Once this occurs, the body's immune system can identify and destroy the cancer cells.
"This part of the cell combination is like a delivery truck," Gu said. "We can package medicines or immune system boosters on the cell surface of platelets, and have them activated to unload once at the target site inside the body."
The second element of the two-cell combination is hematopoietic stem cells, or blood stem cells, which can find their way into the bone marrow through specific chemical signals.
"The hematopoietic stem cells are like a homing signal to the bone marrow," said Quanyin Hu, a lead author of the paper and former doctoral student in Gu's research group. "Once the stem cells guide the combo cells into the marrow, the platelets can be activated. They release immunotherapy cargoes inside the marrow to facilitate the body's own defenses, in this case T cells, to kill leukemia cells."
The researchers plan to continue studying the approach as a potential therapy for leukemia and other diseases.
Quanyin Hu, Wujin Sun, Jinqiang Wang, Huitong Ruan, Xudong Zhang, Yanqi Ye, Song Shen, Chao Wang, Weiyue Lu, Ke Cheng, Gianpietro Dotti, Joshua F. Zeidner, Jun Wang, Zhen Gu. Conjugation of haematopoietic stem cells and platelets decorated with anti-PD-1 antibodies augments anti-leukaemia efficacy. Nature Biomedical Engineering, 2018; DOI: 10.1038/s41551-018-0310-2